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In-Line UV & Turbidity

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PendoTECH is pleased to offer In-line Single-Use UV Absorbance and Turbidity measuring and monitoring tools that collect data from bioprocess fluid streams while it is processing. This results in less disruption to bioprocessing operations compared to measurements taken offline.

PendoTECH’s Single Use UV Flow Cells are coupled with a compact PM2 photometer with fiber optic cables to measure the UV absorbance in filtration and chromatography applications without product contact. Our Turbidity Flow Cells and photometer measure the turbidity of unclarified material from a bioreactor or fermentor, or measure bioprocessing liquid after it flows through a filter to provide consistent monitoring of filter performance.

PendoTECH’s Single Use UV Flow Cells are cost-effective for disposable applications, but may be repeatedly cleaned and reused. Our UV and Turbidity transmitters can be integrated into a monitor with data acquisition capability or a control system, as they are 2-in-1. A dual-wavelength unit is available for increased versatility, and two parallel measurements can be made with one flow cell.

PendoTECH Single Use UV Flow Cells and PM2 Photometer Data Sheet

PendoTECH Turbidity System Data Sheet

PendoTECH Single Use UV Absorbance Sensors
In bioprocess operations, the UV absorbance of a liquid solution can identify either the absence or presence of the molecule of interest. The measurement, typically at 280nm, is made by a spectrophotometer either in-line or off-line in a cuvette. A collimated beam of light passes through a sample with a defined path length and the absorbance is determined as the ratio of the light applied from the source to what passed through the sample. The PendoTECH Single Use UV Flow Cell enables the measurement to be made non-invasively. It is low cost for single use applications and may be repeatedly cleaned and reused. The flow cell is easily integrated to tubing and the measurement is made by use of a compact photometer with fiber optic cables. This flow cell contains a special glass window on the wall and compartments to attach the light source and detector. The stream to be measured flows between the windows by way of tubing attached to the hose barb ends of the flow cell.

Single Use Flow Cell Highlights

  • 1/2 in hose barb fitting; 1 cm path length
  • 1/4 in hose barb fitting; 5 mm path length
  • 1/8 in hose barb fitting; 2 mm path length
  • Unobstructed flow path & no dead legs
  • Qualified for use to 75 psi (5 bar)
  • Compatible with gamma irradiation & autoclavable
  • All polymeric materials meet USP Class VI
  • Each sensor is 100% tested for quality during manufacturing, including leak test
  • Manufactured in a FDA registered, ISO 7 clean room
  • Caustic and chemical resistant polysulfone
  • Lot Certificate of Quality included with each sensor

PendoTECH Turbidity Flow Cell & Measurement Unit
In bioprocess operations, the turbidity of the liquid after a filter can be measured as an indication of filter performance. It can also be used to measure unclarified material directly from a bioreactor or fermentor. The measurement may indicate undesired components are “breaking through” the filter meaning the filter is losing its capacity. The turbidity measurement at 880nm can be used in conjunction with pressure measurements in constant flow filtration processes to give an overall measurement of filter performance. To make an online turbidity measurement, use a device such as the PendoTECH Turbidity Flow Cell versus taking a discrete sample and measuring offline.

Single Use Flow Cell Highlights:

  • Several path lengths available for different turbidity ranges
  • Unobstructed flow path & no dead legs
  • Qualified for use to 75 psi (5 bar)
  • Compatible with gamma irradiation & autoclavable
  • All polymeric materials meet USP Class VI
  • Each sensor is 100% tested for quality during manufacturing, including leak test
  • Manufactured in a FDA registered, ISO 7 clean room
  • Caustic and chemical resistant polysulfone
  • Lot Certificate of Quality included with each sensor


Photometer
PendoTECH® offers a Dual Wavelength version of the PM2 Photometer, which is equipped with two LED light sources. This system is available to output 260nm, 280nm, 300nm or 880nm, and measures two wavelengths simultaneously. Measuring the absorbance of two wavelengths in the same sample has many advantages in biopharmaceutical development and manufacturing. For instance, the user could detect protein, and measure turbidity simultaneously in the same sample by using a system at 280 and 880nm. Likewise, a photometer with two wavelengths in the UV range can detect binary mixtures if the products have different absorption spectra. For example, measuring the absorbance of a sample at 280nm to detect proteins, as well as at 260nm to detect nucleic acids, and then taking the ratio, to provide an estimate of the purity of the solution.

Benefits:
• Dual functionality for lab and benchtop uses or panel mount installations
• Non-invasively measure UV absorbance inline and in real time
• Reliably identify the absence or presence of a molecule of interest
• Monitor changes in concentration and easily determine absorbance peaks
• Low cost for single-use applications, but flow cells are robust enough to be repeatedly cleaned and reused
• Variety of flow cell sizes and path lengths to support different applications and process scales

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