PendoTECH - Data

	Magma Diaphragm Pump: Low Shear Handling of Cells
	To demonstrate the low shear performance of the diaphragm pump in the Magma Advanced Pumping System, two Sf9 (Spodoptera frugiperda) Insect Cell Cultures infected with recombinant baculovirus were compared. One culture was recirculated through the Magma APS diaphragm pump and one was without recirculation. Insect cells become fragile when infected with the virus and require gentle handling.
	Bioreactor Without Recirculation (Control)	Bioreactor With Recirculation with the Magma Advanced Pumping System (6 vessels volumes per hour)

Data: The total cell counts and viability were comparable between the control vessel (no recirculation) and the vessel with the Magma pump recirculating at a rate of 6 vessel volumes per hour. The average cell diameter in the culture pumped with Magma pump with the recirculation tracked higher.

Additional Experimental Information:

The bioreactors were autoclaved sterilized, one with the Magma pump head with two check valves installed on a 5 foot length of ¼ inch ID Pharmed tubing connected to vessel dip tubes on both ends. Each vessel was inoculated with two liters of cells from a shake flask that was infected with the baculovirus 24 hours earlier.
The cultures in the bioreactors were temperature controlled at 27°C and dissolved oxygen was controlled to a minimum set point of 50%. Recirculation of the culture with the Magma APS was commenced at 0.2 liters per minute (6 vessel volumes per hour). The Magma pump head was completing a full cycle every 16 seconds. Samples were immediately drawn from each vessel and tested on a Cedex AS20 analyzer. Subsequent samples were taken during the following 24 hour period. The flow remained precisely at 0.2 liters per minute with no adjustments.

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