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| Cross Flow Filtration- Unidirectional or Reverse Cross Flow (RXF) IDEAL FOR VACCINE AND VIRUS PRODUCTION! |
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| Sanitary and Low Shear Cell Harvest, Media Exchange, and Perfusion with Disposable Processing Options |
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The Magma APS in conjunction with a hollow fiber filter can be used for cell harvest, media exchange, or you can create a bioreactor perfusion system. The hollow fiber filter retains the cells in the bioreactor and the cross flow through the hollow fiber inhibits debris from fouling the membrane filter surface. The low shear pump minimizes cell damage [SEE PUMP PERFORMANCE DATA] and eliminates the use of tubing in a pump that can rupture and spill vessel contents especially during an extended process such as perfusion. The sanitary design maintains bioreactor sterility. In RXF filtration, the flow travels one way inside the hollow fibers, then reverses and flows back in the other direction. In unidirectional cross flow, the flow is only in one direction. The system can also be used to integrity test a filter module. |
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Reverse Cross Flow (RXF) Filtration without Recirculation |
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| When operating RXF filtration without recirculation, once the appropriate filter area required is determined, the pump and pump parameters can be selected to ensure turnover of liquid from the filter and entire vessel connection holdup and the proper shear rate in a fiber. The pump volume must be larger than the filter hold-up volume and the entire vessel connection holdup. The flow rate in and out of the pump must be set to deliver the proper shear rate in the fibers to minimize cell damage but prevent membrane fouling (e.g., low shear is 2000 sec-1). The frequency of cycling of the pump is determined by the flow rate in and out of the pump (cross flow rate). Versus the recirculation mode, this requires a larger pump to achieve equilibration of cells to the vessel and will have less frequent pump cycling to achieve the same cross flow rate. In this mode, there is only one vessel connection required and there is no need for check valves or pinch valves. | |||||
| Unidirectional Cross Flow Filtration with Magma APS | |||||
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In the unidirectional cross flow filtration mode, the low shear diaphragm pump can be used as a recirculation pump where a peristaltic pump is typically used. As shown above, there will be periods of cross flow and no cross flow as the Magma pump draws in liquid and then expels liquid. The "Run Interlock" control from the Magma Controller can stop the filtrate pump when the Magma pump is drawing in liquid so the filtrate pump is not running when there is no active cross flow. In this mode, once the appropriate filter area required is determined, the flow out of the pump must be set to deliver the proper shear rate in the fibers to minimize cell damage but prevent membrane fouling (e.g., low shear is 2000 sec-1). The frequency of cycling of the pump is determined by size of the pump selected. Magma Features to Optimize a Cross Flow Filtration Process The weight control system can be used to maintain the vessel level, the run interlock can shut off the filtrate pump when the system stops to preserve filter capacity, and the external pressure transmitter can be used to monitor filter performance. Please read our Application Note for more information on cross flow filtration and the advantages of using the Magma APS for cross flow filtration. The Magma APS control system can also be used for a pre-use filter integrity test. Please contact us for more information on using the Magma APS for the filter integrity test. |
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Please contact us at request@pendotech.com for more information. Click here to fill out our online form to join our mail list for new product and service updates. Copyright © 2007, by PendoTECH |